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ORIGINAL ARTICLE
Korean J Pediatr 2012 November;55(11) :420-423.
Published online 2012 July 06.        doi:https://doi.org/10.3345/kjp.2012.55.11.420
Regional differences in phylogenetic group of Escherichia coli strains isolated from children with urinary tract infection in Korea
Ui-Yoon Choi (Choi UY)1, Seung Beom Han (Han SB)1, Soo Young Lee (Lee SY)1, Jin Han Kang (Kang JH)1, Sun Mi Kim (Kim SM)2, Sang Hyuk Ma (Ma SM)3
1Department of Pediatrics, The Catholic University of Korea School of Medicine, Seoul, Korea
22Department of Pediatrics, Seoul Adventist Hospital, Seoul, Korea
3Department of Pediatrics, Changwon Fatima Hospital, Changwon, Korea
Corresponding Author: Soo Young Lee ,Tel: +82-32-510-5692, Fax: +82-32-503-9724, Email: sylee@catholic.ac.kr
Copyright © 2012 by The Korean Pediatric Society
ABSTRACT
Purpose: We phylogenetically analyzed the Escherichia coli strains isolated from children with urinary tract infection (UTI) in 2 regions of Korea. Virulence factors (VFs) and antibiotic resistance of the strains were also determined to compare the possible differences. Methods: A total of 138 E. coli strains were collected from the 2 regions; Gyeongin (78 strains) and Gyeongnam (60 strains). The phylogenetic groups were determined using the triplex polymerase chain reaction (PCR) method and multiplex PCRs were used to detect 7 VFs genes (fimH, papC, iutA, hlyA, sfa/focDE, afa/draBC, and kpsMT II). We also tested for antibiotic resistance. Results: Phylogenetic groups, B2 (61.6%) and D (26.8%), comprised the majority of all isolated strains. Regional comparisons revealed that more B2 strains and fewer non-B2 (A+B1+D) strains were found in Gyeongnam, than in the Gyeongin region (P=0.033), and certain VFs were predominantly detected in Gyeongnam (P<0.05). Neither regional nor phylogenetic differences, in antibiotic resistance of the strains, were significant. Conclusion: We were able to confirm that the geographic location is an important determinant of the distribution of the phylogenetic groups and VFs among the E. coli strains that cause UTI in children.
Keywords: Urinary tract infections | Escherichia coli | Phylogeny | Child
 
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