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ORIGINAL ARTICLE
Korean J Pediatr 2012 November;55(11) :424-429.
Published online 2012 July 23.        doi:https://doi.org/10.3345/kjp.2012.55.11.424
Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction
Jong Gyun Ahn (Ahn JG)1,2, Seong Yeol Choi (Choi SY)2, Dong Soo Kim (Kim DS)2,3, Ki Hwan Kim (Kim KH)2,3
1,2Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul, Korea
2,3Department of Pediatrics, Severance Childrens Hospital, Yonsei University College of Medicine, Seoul, Korea
2Department of Medicine, The Graduate School of Yonsei University, Seoul, Korea
Corresponding Author: Ki Hwan Kim ,Tel: +82-2-2228-2050, Fax: +82-2-393-9118, Email: khkim99@yuhs.ac
Copyright © 2012 by The Korean Pediatric Society
ABSTRACT
Purpose: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient than conventional culture in achieving S. pneumoniae -positive results. Methods: Nasopharyngeal (NP) secretions were obtained from 842 pediatric patients admitted with lower respiratory infections at Severance Childrens Hospital in Korea between March 2009 and June 2010. For identification and serotype determination of pneumococci from the NP secretions, the secretions were evaluated via multiplex PCR technique with 35 serotype-specific primers arranged in 8 multiplex PCR sets and conventional bacteriological culture technique. Results: Among the results for 793 samples that underwent both bacterial culture and PCR analysis for pneumococcal detection, 153 (19.3%) results obtained by PCR and 81 (10.2%) results obtained by conventional culture technique were positive for S. pneumoniae. The predominant serotypes observed, in order of decreasing frequency, were 19A (23%), 6A/B (16%), 19F (11%), 15B/C (5%), 15A (5%), and 11A (4%); further, 26% of the isolates were non-typeable. Conclusion: As opposed to conventional bacteriological tests, PCR analysis can accurately and rapidly identify pneumococcal serotypes.
Keywords: Streptococcus pneumoniae | Multiplex polymerase chain reaction | Culture
 
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