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Korean J Pediatr 2012 December;55(12) :474-480.
Published online 2012 November 02.        doi:
Evaluation of immunogenicity of the 2008–2009 seasonal influenza vaccines by microneutralization test
Seung Youn Kim (Kim SY)1, Yun Kyung Kim (Kim YK)2, Byung Wook Eun (Eun BW)3, Nam Hee Kim (Kim NH)4, Eun Kyeong Kang (Kang EK)5, Byong Sop Lee (Lee BS)6, Jung Sub Lim (Lim JS)1, Jun Ah Lee (Lee JA)1, Dong Ho Kim (Kim DH)1
1Department of Pediatrics, Korean Cancer Center Hospital, Seoul, Korea
2Department of Pediatrics, Korea University College of Medicine, Seoul, Korea
3Department of Pediatrics, Graduate School of Medicine, Gachon University of Medicine and Science, Incheon, Korea
4Department of Pediatrics, Inje University College of Medicine, Ilsan Paik Hospital, Goyang, Korea
5Department of Pediatrics, Dongguk University Ilsan Hospital, Goyang-si, Korea, Goyang, Korea
6Department of Pediatrics, Asan Medical Center, Childrens Hospital, University of Ulsan College of Medicine, Seoul, Korea
Corresponding Author: Dong Ho Kim ,Tel: +82-2-970-1298, Fax: +82-2-970-1970, Email:
Copyright © 2012 by The Korean Pediatric Society
Purpose: For evaluating the immunogenicity of an influenza vaccine, the microneutralization (MN) test has a higher sensitivity and specificity as compared to the hemagglutination inhibition (HI) test. However, the MN test is more time consuming and is difficult to standardize. We performed the MN test to determine its usefulness as an alternative or complementary test to the HI test for evaluating the immunogenicity of influenza vaccines. Methods: We compared the MN test with the HI test using 50 paired samples taken from a previous clinical study (2008–2009) in Korean children under 18 years of age. Results: The linear correlation coefficients of the 2 tests for H3N2, H1N1, and influenza B were 0.69, 0.70, and 0.66, respectively. We identified a high index of coincidence between the 2 tests. For an influenza vaccine, the postvaccination seroprotection rates and seroconversion rates determined by the MN test were 78.0% and 96.0%, 90% and 42.0%, and 42.0% and 48.0% for H3N2, H1N1, and influenza B, respectively. Geometric mean titer fold increases of H3N2, H1N1, and influenza B were 2.89, 5.04, and 4.29, respectively, and were 2.5-fold higher. We obtained good results in the evaluation of the immunogenicity of the 2008–2009 seasonal influenza vaccines. Conclusion: We found that the MN test was as effective as the HI test. Therefore, we suggest that the MN test can be used as an alternative or complementary test to the HI test for evaluating the immunogenicity of influenza vaccines.
Keywords: Neutralization tests | Hemagglutination inhibition test | Influenza vaccines | Child
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